Tory Burch Outlet Chondrocyte isolation and cultur

43nike9485qt · Wysłany: Pon 21:47, 21 Mar 2011

Chondrocyte Isolation and Culture

Need a variety of enzymatic digestion , increasing the possibility of cell contamination . Our approach is alone in stages Ⅱ collagenase digestion ,[link widoczny dla zalogowanych], each digested 45min, collected a cell , and as quickly as possible additional culture medium containing serum to terminate digestion, and protection of culture medium plus serum . After two 45min basically completely harvested digested cells. Then the cells together to digest the second count , sub- flasks . Because the contact time is too long collagenase toxic effects on chondrocytes , it is not a digest 2 ~ 3h , or 5-7h before harvesting cells. And most of the cartilage cells,[link widoczny dla zalogowanych], rabbit chondrocytes optimum pH value of 7.4 , so always check the pH value of culture medium , such as the time for medium to medium yellow . Medium placed in the refrigerator after saving often alkaline , use hydrochloric acid to adjust . In chondrocytes cultured microwave biological effects and research progress Luen Yong Xu Xiangmin LAI Sheng- Li South China University of Telecommunications Institute ( Guangzhou 510640, China ) Guangzhou Pharmaceutical in 2005 36 No. 1 in the process is always to prevent pollution, attention aseptic cartilage cells in vitro Training process , there are two outcomes : First,[link widoczny dla zalogowanych], continue to maintain phenotype , with secretion of type Ⅱ collagen fibers and chondroitin sulfate proteoglycans (CSPG) capacity ; second place anti- differentiation, cell shape changes only , and the secretion of collagen Type Ⅱ fiber is no longer to become I- , CSPG synthesis capacity also will be lost. We are based on identification of chondrocyte phenotype , that within three generations of high activity of cartilage cells , cartilage cells to maintain phenotype, is ideal for experimental study cells. After three generations of anti- cartilage cell differentiation occurs gradually , cell volume increases , there are many finger-like projections , the nucleus increases,[link widoczny dla zalogowanych], the cell proliferation slowed down as a cartilage degenerative changes in the research model . So the best response to the study drug and the best selection of tissue-engineered cartilage chondrocytes within three generations .
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