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asbryobvrz · Wysłany: Nie 12:21, 20 Mar 2011

Physical and mental condition of patients affected by paraplegia Investigation of the Influence of family factors

. Hegang City People's Hospital of Heilongjiang 154100) (2. Jiamusi University, 154007) 1 1.1 Materials and methods Drugs and reagents streptozotocin, Sigma; 125I an insulin radioimmunoassay kit (China Institute of Atomic Energy Isotope Institute); glucose liquid kit (Weifang 3V Bioengineering Co.,[link widoczny dla zalogowanych], Ltd.). Malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), nitric oxide synthase (NOS) kit for the products of Nanjing Jiancheng Bioengineering Institute,[link widoczny dla zalogowanych], Harbin, berberine were purchased from three refined pharmaceutical companies. 1.2 experimental rat model of type 2 diabetes and group preparation of Wistar 30 rats, weighing 210 ~ 250g, by the Experimental Animal Center of Jiamusi University, fasting before the test 16 ~ 18h (free water), 20 were randomly selected by the intravenous injection of STZ (When you use this to 0.1mol / L, pH value of 4.4, a citrate buffer solution of sodium citrate 2% STZ solution dubbed) 25mg/kg,[link widoczny dla zalogowanych], the remaining eight tail vein for the normal control group injecting the same amount of a citric acid sodium citrate buffer. Normal diet 2 weeks after injection, the glucose tolerance test, the selection of impaired glucose tolerance (IGT) a total of 16 rats were randomly divided into model group and berberine treatment group, n = 8, fed a high fat high sugar diet, the basal diet plus sucrose, refined lard, a mixture of fresh eggs, the total energy 20.1J / g (protein 15%, 51% carbohydrate, fat 25%); normal control group was fed the basic diet. Berberine berberine group were fed daily liquid 0.1g/kg? D, the normal group and diabetic group fed same amount of tap water per day, continuous feed and fed for 12 weeks. Done the day before the end of the experiment glucose tolerance test. 1.3 Observation of indicators and methods 1.3.1 General Project glucose with glucose oxidase. Serum insulin by radioimmunoassay. Experimental measurement by radioimmunoassay of urinary albumin 4,8,12 weeks. Lipids with AU640 automatic biochemical analyzer. 1.3.2 Preparation of testicular tissue MDA, NO levels,[link widoczny dla zalogowanych], SOD, NOS activity was measured after ether inhalation anesthesia cut the scrotum, exposing the testicles, taking the right testis at 4 ℃,[link widoczny dla zalogowanych], washed in saline pre-cooling the blood, After weighing the filter paper drain water, per gram of testicular tissue homogenate plus 9mL saline set-top glass homogenized about 3 ~ 4min, 10% of testicular tissue preparation. MDA (malondialdehyde, MDA) content by thiobarbituric acid method, superoxide dismutase (superoxidedismutase, SOD) activity with xanthine oxidase, nitric oxide synthase (nitricoxideynthase, NOS) activity compared with the chemical Determination of color, NO content determination of NO2 with a nitrate reductase / NO3 indirectly reflect a content, determined by Coomassie brilliant blue protein. Kits were purchased from Nanjing Jiancheng Bioengineering Institute SPSS10.0 1.3.3 statistical methods used statistical software for statistical analysis, each set of experimental data are mean ± standard deviation (X ± S), said group Difference between the mean number of completely random data into a group t test. 2 Results 2.1 Comparison of blood glucose insulin diabetic group before the trial and treatment group compared with the control group there were significantly abnormal glucose tolerance (P <0.01), that model was successfully. End of the trial compared with the control group, blood glucose values of diabetic rats significantly increased (P <0.05), serum insulin decreased significantly (P <0.01). Berberine treatment group compared with the diabetic rats, blood glucose decreased significantly (P <O.05), serum insulin levels (P <O.05). Table 1 test blood glucose before the start of the level of each group (±, mmo1 / L, n - Cool

P <O. 0l (compared with the control group) at the end of Table 2 test the blood glucose and insulin levels (≤, mmo1 / L, n- Cool

· P <0.01 (compared with the diabetic group), P <O. 05 (compared with the diabetic group) 2.2 Biochemical testis testis of diabetic rats, the level of MDA, NO levels and NOS activity were higher (P <0.05). Compared with the diabetic group, MDA levels of berberine treatment group (P <0.01), NO levels, NOS activity (P <O.05) were significantly higher while SOD activity decreased (P <0.05), close to the control group. -