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ORANGE EKSTRAKLASA
Dołączył: 03 Mar 2011
Posty: 720
Przeczytał: 0 tematów
Ostrzeżeń: 0/5
Skąd: England
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 Wysłany: Czw 15:40, 17 Mar 2011 |
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,[link widoczny dla zalogowanych]
Cobra venom L- amino acid oxidase purification and inhibition of vascular endothelial cells
oandinvivo [J]. ActaBiochimBiophys,[link widoczny dla zalogowanych], 2008,40 (1) :1-6. [ 10] Long Miao Yun, Qi-Chang Zheng , Song Zifang . Inhibition of angiogenesis inhibitor arresten hu-vec cells proliferation and migration of experimental study [J]. Pharmacol , 2007,23 (1 ) :64 -6. [10] LongMY,[link widoczny dla zalogowanych], ZhengQC, SongZF. Effectsofarrestenonhuveccellproliferationandmigration [JJ. ChinPharmacoBull, 2007,23 (1) :64-6. PurificationofL-aminoacidoxidasefromNajaatravenomanditseffectsonendothelialcellsCHENZhou,[link widoczny dla zalogowanych], HUANGJian-jun, XUELing, XUYun-lu. , LINLi. shan. (1.CollegeofPharmacy, 2.SchoolofElementaryMedicine, FujianMedicalUniversity, Fuzhou350004, China; 3.DeptofPharmacyofPutianCollege, PutianFujian351100) Abstract: AimTopurifyL-aminoacidoxidase (LAAO) fromthevenomofNajaatraandstudyitseffectonendothelialcells. MethodsTheNAV-LAAOwaspurifiedbyion-exchangechromatographyandaf-finitychromatography. TheM1TrassayandWesternblotwereusedtodetecttheviabilityandapoptosisofHU-VEC. Thetubule-formingwasusedtostudytheangio-genesisofcells. ResultsTheNAV-LAAOwaspuri-fledsuccessfullyfromthevenomofNajaatra. Theno-lecularweightofNAV. LAAOwasdeterminedtobe58kubvSDS. PAGE. NAV-LAAOeffectivelyinhibitedthegrowthandtubule-formingofHUVEC,[link widoczny dla zalogowanych], andthe50% inhibitoryconcentration (IC5o) was21.42mg · L ~. Comparedwithcontro1. thelevelsofcaspase-3andcaspase - 8increasedinHUVECtreatedwithNAV - LAAO. ConclusionsTheNAV-LAAOispurifiedsue-cessfullyfromNajaatravenombyion-exchangechro-matographyandaffinitychromatography. TheNAV-LAAOinhibitsthegrowthandtubule-formingcapacityofHUVECinadose-dependentmanner. Keywords: Najaatravenom; L-aminoacidoxidase; purification; HUVEC; endothelialcells / tubule · formingcapacity; Affinitychromatography
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