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yxfbbiedtj · Wysłany: Wto 4:48, 08 Mar 2011

System of patients with bronchial asthma preliminary assessment of the effect of education

Materials and Methods: 12 RA patients fell, of which 9 females, 3 males fell, the average age of 41.9 ± 7.8 years; vines down 5 OA patients,[link widoczny dla zalogowanych], female 4,[link widoczny dla zalogowanych], and M 1 side, with an average age of 49.8 ~ 5.7 years old. Diagnosis of RA and OA are consistent with American College of Rheumatology Association standards set by Pina. Vine specimens from patients with RA and OA synovial fluid analytes ET; elect confirmed RA8 7 women down, men and 1 down, the average age of 46.9 ± 6.3 years, whichever is the surgical removal of synovial specimens cultured synovial cells isolated and reagents: Full RPM [1640 (with 10 fetal calf serum and penicillin and streptomycin) f collagenase type I (a product) t using the concentration of lg / L. ET measured with radioimmunoassay reagents and methods [1]. Synovial cell isolation and culture: The synovial membrane was cut and digested by the cavity of the original,[link widoczny dla zalogowanych], and then filtered into sterile gauze ten single cell suspension, centrifuged RPMI with 10 fetal calf serum medium alone dubbed resuspended Fan liquid ten cells (trypan blue exclusion test holding cell viability measured at 95 and above). Purchase 96-well culture plates (1 × lO ten cells / well) culture (37 ℃ .5 COz) 48 hours, collect supernatant stored in a 20 Results in RA and OA synovial fluid levels of ET measured by radioimmunoassay showed that in RA synovial fluid l2 down 9 cases detected in the ET1. The average content of 4.9 ± 3.4ng / L (±)} in 5 patients, 2 back down OA measured ET, contents were 26.4 and 18.9ng / L. The chi-square test, and oA ET fish out rate between groups, P> 0.05. RA patients with the same determination in synovial cell culture supernatant levels of ET showed that the 8 down in 4 cases can be measured to the ET, its content ranges from 3.1 ± 1.1ng / L (cover disabilities), and the other 4 cases not detected ET1. Author t100730 China Academy of Medical Sciences Peking Union Medical College Hospital, Peking Union Medical Howe discussed the North is known dig ET There are 3 different body, that is, ET,[link widoczny dla zalogowanych], E and ET;. ET is a peptide of 21 amino acids, and its main function is to stimulate the contraction of blood vessels and vascular smooth muscle cell proliferation in some of our patients with RA and OA in the measured slip Lang ET, which Nahir, etc.: basically the same report, the authors reported RA ET levels in synovial fluid 2.5 ± 0.4ng / L (± Island), OA synovial fluid in the ET1 was 3.0 ± 0.5ng / L in persons, such as), the difference was not significant. Another ~ b; Miyasaka, are also reported RA and OA synovial fluid levels of ET were no significant differences,[link widoczny dla zalogowanych], RA the ET concentration was 22.85 ± range of anonymous 4.63ng / L (j disabilities, such as.) However, control over their own levels of serum, and the active than inactive. The synovium of RA is also measured in the supernatants of ET, which we poured in 8 cultured synovial cells detected in 4 cases consistent with the ET. 2 cases of OA in this group higher than the RA synovial fluid in patients with ET may be a few steps with the disease side, and the sampling of various related diseases. Measured in RA and OA tips ET1, endothelin is not only a vasoconstrictor and may have other effects. It was recently reported that synovial macrophage-like cells also produce ET. And ET can stimulate fibroblast proliferation} human umbilical vein endothelial cell culture medium can also be detected in the ET, and endothelial cells play an important role in RA. The study suggests, ET may be involved in the pathogenesis of some rheumatoid arthritis, but its mechanism of action is not yet clear Pina, need to further expand the diseases and cases of serum, smooth synovial membrane culture supernatants possession and ET levels, which may help to reveal the ET in rheumatic Tanbing such as the significance of RA