A chlorine ether on peripheral blood lymphocytes i

yxfbbiedtj · Wysłany: Pią 18:25, 25 Mar 2011

A chlorine ether on peripheral blood lymphocytes of workers exposed to the influence of chromosome

(103) [Key words] Cl A ether; contact; lymphocyte chromosome [CLC] R1352 [Document code] A [Article ID] 1006-7655 (2001) 05-0724-01 chloroformate ether is a chemical intermediate, is widely recognized as carcinogenic factors in the production and use of ether in contact with chlorine can cause a higher incidence of lung cancer among exposed. A further understanding of chlorine on human health effects of ether, to explore the carcinogenic mechanism. Explore the population exposed to biological indicators of early health care, exposure to chlorine in the production process workers, a peripheral blood lymphocyte chromosome ether were investigated. The results reported below. An object and methods 1. i survey a selected chemical synthesis of ether chloride feed line workers, the reactor operator, unloading Packers, handling transport workers and management staff of 53 people supporting the survey. 1.2 The survey methodology survey research subjects occupational history, time, past medical history, medication history. Peripheral venous blood to take the survey, adding l% PHA0.2m】 incubation at 37 48 ~ 72h, 3.5h before harvesting by adding 0.040, l, ag / ml colchicine, absorb the supernatant, add 0 .075 mol / 1 of KCl4m】 20 ~ 30min after the hypotonic, add 1m】 pre-fixative fixed. After centrifugation, fixed, made friends with the original cell suspension culture medium, the system specimen slide, Gienasa staining. Select the cells under a microscope to observe the integrity of chromosome number and structure of 2 Results 2.1 survey survey the general situation of ether in a production line of action of chlorine have worked an average of 2.6 years (14 ~ 3 years), the working time of 7d / w, on-site operation time of 8h / d. All the recent survey were not teratogenic, mutagenic drug use history. Survey of 22 chromosomes observed in the distortion of human chromosome 5. Aberration was 10.4%, mainly chromosomal aberrations workers unloading and transport workers, the specific results in Table l. Distortion in the event of chromosome 5 in human peripheral blood lymphocytes, observed in hyperdiploid and hypodiploid cells,[link widoczny dla zalogowanych], chromosome aberration types are the number of aberrations, the survey found no structural chromosome aberrations (Table 2) . 3 Discussion by epidemiological investigation and laboratory analysis, chlorine ether has been recognized as a carcinogenic factor. induced variation, the results of the survey of workers exposed chloroformate ether appeared the number of peripheral blood lymphocyte chromosome aberration, which also demonstrated from a chlorine ether on a teratogenic effect of human chromosome 1 and Table A chlorine ether number of chromosomal aberrations in workers exposed to chlorine in Table 2 ether A chromosome aberrations in peripheral blood in workers exposed to the carcinogenic effects of body type distortion of the jobs concentrated in the discharge packaging and handling transportation workers, a phenomenon with both types of work A product of chlorine exposure to more opportunities for basic ether is consistent with the general toxic effect on human health dose-response relationship is consistent. The results of the survey the number of chromosomal aberrations mainly distortion, indicating that chlorine in a ether M cell cycle toxic effects on the chromosome, is likely to be the normal cell spindle structure or function of impact damaged the ends of chromosomes to the normal separation of cells, affecting the normal cell division process. Through this survey shows that chlorine in the future health of a population exposed to ether monitoring should be considered in the peripheral blood lymphocyte chromosome the use of biological indicators, can be observed early chloroformate ether on the human body. Since this poll survey exposed the subjects to the short time chloroformate ether, the number of subjects, not many chloroformate to understand the impact of ether on human chromosomes, more to be done further investigation into the ~. f