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II LIGA
Dołączył: 21 Lut 2011
Posty: 227
Przeczytał: 0 tematów
Ostrzeżeń: 0/5
Skąd: England
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 Wysłany: Pią 11:13, 25 Mar 2011 |
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HBV感染者外周血单个核细胞中HBV DNA的检测
a'ng，110001)AbstractObjeclive：ThispaperwastostudythepresenceofHBVDNAinmononuc]earcellsfromperipheralblood(PBMCs)amongsub~etsinfectedwithhepatitisBvirusanditsrelationshipwithserumHBVDNA．Methods：PBMCsandserumsampleswereexaminedbyPCRandDot-blothybridiza-tlonin50patientswithHBsAg(+)inscruff1．ResultslByPCR，HBVDNAwetsdetectedinPBMCsfrom35of50patients(70)．Bydot-blothybridization，wefoundHBVDNAin32(64．O)．Withre-specttodifferenttypesofclinicalHepatitisB，thepositiverateofHBVDNAinPBMCswas60．0．ThepositiverateofHBVDNAin~elxlmwas36．O．Coa~lmdons}HBVDNAcouldexistinPBMC．sin-dependently．WefoundnoobviousdifferenceinthepositiverateofHBVDNAfromPBMCsamongallkindsofclinicalhepatitisB．Keywordsperipheralbloodmononuclearcells{HBVDNA{PCRIdot—blothybridization(上接第561页)MutationsintheExon9ofFGFR2GeneinCrouzonSyndromeMaHongwei，WangYang，MiZheng，etal(GeneticLaboratory，TheSecondClink'MCollegeChinaMedicalUniversity．Shenyang，110003AbstractObjective：Thispaperw0．stounderstandthemolecularpathogenesisofCrouzonsyn—drome．Methods：Westudiedthemutations|nex0n9oftheFGFR2genein6￡arallieswithCr叫∞nsyndrome，usingthemethodofPCR-SSCPandproductsdirectsequencing．Resalts：Thechangesofsin-glestrandconformationa]polymorphismbandshiftwerefoundin5Cases．PCRproductdirectsequenceanalysesshowed4differentmutations，wiihonenovelfinding(Cys342Trpl，Thefrequencyofmitt3-tionatcodon342wasrelativelyhigh．Conclusions：ThemutationofFGFR2genewasconsideredtohethecauseo{thesyndrome．KeywordsCrouzonsyndrome4flbroblastgrowthfactorreceptor2gene·570·
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