Test for coagulase rabbit plasma preparation and

43nike9485qt · Wysłany: Pią 23:36, 11 Mar 2011

Test for coagulase rabbit plasma preparation and storage methods

aiJofMedLabSci2002, Vol17N () 5 A, 3 compared to latex reagent method with the standard biochemical reactions, 54 strains of bacteria, rabbit plasma and was 100% Latex. False positive rate in plasma of patients 12.5% (5 / 40), the false negative rate of 21.4% (3 / 14). Second, the temperature of a 20. C and a 85. C storage in rabbit plasma the best, to 6 months, the results remain unchanged, the effect of longer storage remains to be seen. 2 ~ 8. C storage of plasma 45d failure, manifested as plasma turbidity, fibrin clot with a small amount of precipitation that if the clot singled out, continue to be placed, another clot gradually precipitated. Increased precipitation of fibrin, plasma failure. Lower storage temperature, storage time of coagulation factor activity the longer the better. Hu Jihong, etc. discussed in 1999 and 2000, external quality assessment in clinical microbiology and quality control of the meeting summary mentioned: Quality Assessment of clinical microbiology in the country over the years results in the accuracy of coagulase test has not solved the problem lies coagulase test quality control. We believe that, in addition, many units of convenience plasma of patients treated with plasma or blood banks, but also affect the outcome of important reasons. Inappropriate because of the use of plasma, even if doing quality control, the results may not be accurate. Plasma of patients treated with plasma or blood bank, the following aspects can affect the experimental results: the plasma of patients with a variety of antibodies, drugs; some patients low plasma fibrinogen levels; the impact of different anticoagulants; patients with improper storage of plasma , not in full bloom and a sterile pollution. In addition, if the improper operation, as well as infected with hepatitis B, HIV, and dangerous. Import latex reagents are expensive, difficult to spread. We introduce the method used, a 2.5kg rabbit, at least get the plasma 30m1, were prepared by diluting frozen plasma 300, enough to use a longer time. Remove from the refrigerator to use direct application, without dilution, time-saving convenience.
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