asbryobvrz
ORANGE EKSTRAKLASA
Dołączył: 21 Lut 2011
Posty: 441
Przeczytał: 0 tematów
Ostrzeżeń: 0/5
Skąd: England
|
 Wysłany: Śro 7:22, 09 Mar 2011 |
|
|
,[link widoczny dla zalogowanych]
Adenovirus-mediated LacZ gene expression in the rat sciatic nerve
Efficiently infected a wide range of cell types and become invivo gene therapy an attractive and highly promising carriers, but the body of the adenovirus vector produced antigen-specific cellular and humoral immune response to adenovirus-mediated exogenous not long-term gene expression, thus affecting adenoviral vector gene therapy results. Use of immunosuppressive agents on immune responses and other methods to reconcile the modified adenovirus can reduce the host immune response and prolong expression of foreign genes in time, thus radically improve the effect of adenoviral gene therapy [,[link widoczny dla zalogowanych],. Some people use non-viral vector directly to the foreign gene transfer to peripheral nerve and expressed. Zhang Shiqiang other _8J LacZ gene by directly injected into the rabbit sciatic nerve vector or application LacZ gene plasmid soaked suture medical solution,[link widoczny dla zalogowanych], rabbit sciatic nerve suture observed exogenous gene can be directly transferred to the peripheral nerve in vivo and expression. But the plasmid DNA into the mechanism of target cells is not clear,[link widoczny dla zalogowanych], probably due to the injection process undermines the integrity of local tissue membrane plasmid DNA caused by the intake. This study found that adenovirus-mediated LacZ gene can be transferred efficiently in live animals and the expression of the sciatic nerve SCs,[link widoczny dla zalogowanych], indicating that adenovirus-mediated neurotrophic factor gene therapy for peripheral nerve regeneration. '
Post został pochwalony 0 razy
|
|
