scarpe nike 2011 Gastric smooth muscle tumor cell

xp574088 · Wysłany: Śro 15:24, 09 Mar 2011

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Gastric smooth muscle tumor cell nuclear DNA flow cytometry spectrophotometry

flowcytometry. FCM) quantitative analysis of DNA content in gastric smooth muscle tumors to investigate the molecular level, from DNA and its biological properties. Materials and methods Author: 050011 {be the fourth North Medical Surgical Hospital impaired even cut out items for a male Acai Feng Du Du Ma Zhixue led by CITIC Korean stars like Derek Wang Xiaoling): {to Northern Province, Institute of swollen bundle of malaria Cellular Vision Note Grenfell Hanjian Wen Hui Jiang cut) two Chinese Journal of Digestion August 1995 Volume 4 No. l5 one. Samples from fine flat stomach surgery paraffin-embedded specimens 3o feed fibroids, other 8 cases of normal gastric specimens for flat fine muscle control. Second,[link widoczny dla zalogowanych], single cell suspension prepared from each paraffin tissue blocks were cut 40 microns thick tissue slices 3 to 5, with xylene dewaxing, hydration,[link widoczny dla zalogowanych], and decreasing concentrations of ethanol Yan distilled water rinsing,[link widoczny dla zalogowanych], rub with the legal system or network collagenase the sample. 260 mesh sieve filtration. Collect the filtrate (single cell suspension reservoir). Pyridine bromide has been used (EB), staining for 30 minutes, measured on the machine. Third, conventional histological biopsy HE staining, microscopy emblem was divided into three groups. 8 cases of normal gastric smooth muscle. L6 leiomyoma feeding. Smooth spider guanidine sarcoma 14. IV. Spindle cells detected by DNA FACS420 fluorescent activated cell sorting only. CV value adjustment of the instrument about 5. Test results by computer application software processing. The DNA index (DI), said DNA content, DI; sample cell G. / G. ÷ peak value of normal cells are Road G. / G. Peak to average channel value. By DI = O. 86 ~ 1.15 as a diploid or near diploid. This range is aneuploid. Statistical analysis of the data. Results of one set of DI and the ploidy level distribution of the results show DNA content of sperm myoma group ship no difference with the control group. Table l shows three sets of DI and ploidy distribution. Table 1 normal helmet organization. Leiomyoma. Fort ± plastic vertical dust falling into the plastic sub-limonene cancer and normal smooth muscle,[link widoczny dla zalogowanych], compared with P <0.01 II, stomach cancer, smooth muscle and smooth muscle sarcoma tumor size and the relationship between DI and ploidy in Table 2. Third, fine muscle tumors of the stomach flat and ploidy distribution of DI transfers and prognosis. The group of l4 cases with lymph node metastasis in 2 down, 1 case of widespread metastatic abdominal cavity. L cases of liver metastasis. The distribution of D1 L47, L40, 1. ad alliance L32 are aneuploid. 13 down survival of the ship and the relationship between DI and ploidy in Table 3. Studies show that the nuclear DNA content of the discussion as a malignant abnormal swelling 245. Table 2 Gastric leiomyoma and leiomyosarcoma looking blue plastic dust! Ink ink points falling into the limonene limonene -::! 0.05 ± Yi Xuan Dan plastic plug plastic Wong Yi Wong Fort Fort limonene limonene plastic ink powder deposit on the back -:: Blue <3 years 61.30 ~ 1A7l3B disabilities O. 0606 marks an important tumor may be active on their growth. gastric smooth muscle tumor group was significantly higher than DI ships and smooth muscle tumor group. and 78.57 were aneuploid distribution. with the reported similar The results show that in this group. 4 had peritoneal metastasis of DNA content and ploidy distribution were abnormal. In addition. 3-year survival group, DNA content was less than 3 years and 3 years or more groups. And the former was 100% aneuploid, the latter study only. 14 hatch. The reason may be ravioli are: high volume or DNA units in the aneuploid tumors there is a large number of unpaired chromosomes, leading to metabolic imbalance more pronounced and edge split. Accelerate cell proliferation. More unstable combination of genes and showed a higher mutation rate. These changes may enhance the ship tumor cell invasion, metastasis and recurrence more likely to occur. Shortened survival.
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