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Bacterial contamination of air in the bus survey
Bus sampling. 1.4 Preparation of sample preparation and sampling of bacterial culture dish for adequate access, natural Shen Received Date :2003-04-15 Author: Dawn Wong (1967 a),[link widoczny dla zalogowanych], female, Technician, Research Interests: microbiology and cell Biology 5l Guizhou Normal University (Natural Science) Volume 21 sample products drop method, the sample plate before mounting the sample inside the rear compartment from the high end of 1.5rll, open culture plate cover, vaccination 10min, while Record number of people on the bus. Each part of the three mining pan, the mean value. Were placed in experimental inoculation of the sample plate rack room, 1.5m high from the ground, open the Petri dish cover vaccination 10min, covered with pan lid, inoculation room no. 1.5 culture and count samples of bacteria after inoculation release plate incubator, 37 ℃ cultured 48h, the number of bacterial colony counts, count results in Table 1. 1.6 Gram staining and microscopy Graft cannot choose to represent the colony Training for Gram stain, microscopic examination. Pollution inside the bus that bacteria, Bacillus bacteria than, G than G-. Air bacterial concentration of 1.7 formula (Austria's formula) C = 5O00N / A. T where: c said that the total number of bacteria per cubic meter of air contains (bacteria of the time (min); 2 Results and discussion 2.1 bus bacteria within the air pollution survey (see Table 1) Table 1 shows that the concentration of bacteria pollution inside the bus in the population density (5 / m) when , January (winter) the highest average of up to 22,319 / m, compared with the control average of 873 / m 25 times. In the same population density,[link widoczny dla zalogowanych], in April (spring), October (autumn) of air pollution inside the bacterial concentration decreased, averaging 12086 + / m,[link widoczny dla zalogowanych], 14399 个 / m, compared with the control mean 699 / m, 882 feet / m higher than the 17.2 times and 16.3 times in July (summer) air pollution inside the bacterial concentration at least, 5685 / m, compared with the control 722 feet / m higher than 7.8 times. The population density of 0.5 people / m, the bacteria inside the concentration of air pollution has declined. January (winter), causing serious air pollution inside the densely populated than the causes, the cold weather, inside all the windows closed and ventilation is an important cause of bacteria causes serious pollution. April (spring), October (autumn) of air inside the bus in the population density is high are more serious bacterial contamination, but some windows open, air circulation is better, so the concentration of bacteria in the air pollution decrease. July (summer) due to hot weather, all the windows and inside the top window open, good air circulation, so the concentration of bacterial contamination of the air is relatively small. 52 former Soviet air health bacteriology loan evaluation criteria, total number of bacteria is greater than 7000 feet in winter / m can be regarded as air pollution, and crowded bus when the concentration of bacteria has reached 22,319 / m,[link widoczny dla zalogowanych], shall be deemed to bacterial contamination of the air inside more serious. Many scholars believe that China's current can be greater than the total number of bacteria in indoor air with 1000 / m of air pollution as an evaluation index J. According to cell morphology confirmed that air pollution inside the bus than bacillus bacteria, G G more than one respiratory infection caused by bacteria and pathogens class is consistent. Bus bacterial contamination of the air inside the main reason is the population density inside too, caused by poor ventilation,[link widoczny dla zalogowanych], the proposed anti-labor department of health to strengthen health prevention on the bus and supervision of labor, to prevent the spread of respiratory disease has a very important significance. Table 1 Bacteria Levels of air pollution, bus tables (2001) Note: I said the population density of about 5 / m of air in the colonies (claw / m); Ⅱ that population density is about 0.5 people / m in air the colonies (a / m).
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