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Wysłany: Pon 12:14, 17 Sty 2011
Temat postu: tory burch outlet Hypoxia promotes cardiac fibrobl
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Hypoxia promotes cardiac fibroblast DNA synthesis and Ⅰ, Ⅲ collagen mRNA expression
ter6h, 12hhypoxia (2% o2) exposurerespectively. Thelevelofpro-~ / [(I) collagenmRNAexpressionwassignificantlyelevatedinthecellsunderhypoxiafor4h, 8h, and12h. Theexpressionofpro-al (11I) mRNAincreasedwhencellswereculturedunderhypoixafor2h. Conclusion: TheseresultssuggestthathypoxiaalonecanupregulateDNAsynthesisandexpres. sionofcollagentypeIand11ImRNAinadultratcardiacfibroblsts. ItmaybeoneoftheimportantmechanismywhichhypoxicmyocardialfibrosisOccur. KEYWORDS: hypoxia; cardiacfibroblasts; DNAsynthesis; collagen; rat organophosphate insecticide chlorpyrifos and the cooling effect caused by changes in blood pressure recorded Yang, Yang Zhen, Li Yushan (1. Bayi Institute of Physiology, Lanzhou Medical College, Lanzhou 730020, China; 2. PLA 29 hospitals, Tianshui, Gansu 741020) Abstract Objective: To observe the organophosphate insecticide poisoning cicada (CUP) and the cooling effect caused by the relationship between blood pressure. Methods: Rats with wireless telemetry technology for continuous measurement of body temperature and blood pressure changes were observed feeding CHP on oral temperature and blood pressure. Results: The rats were given oral feeding CHP30mg/kg lower body temperature response is obvious, but significantly increased blood pressure, the phenomenon appeared, 12h after the administration of body temperature and blood pressure returned to previous levels. Conclusion: Organic phosphorus pesticides ClIP induced cooling in the process, accompanied by the phenomenon of blood pressure. Key words: organophosphorus pesticides; temperature; blood pressure KEYWORDS: organopho ~ phatepesticides; bodytemperature; bloodpressure CLC number: 482.2 Document code: A Article ID: 1O00-6834 {2004) 02-0128. 02 in animals studies have found that anti-cholinesterase agents to regulate temperature can cause significant disturbance to the animal adjacent neostigmine, physostigmine and organophosphate pesticides can cause hypothermia. Anti-cholinesterase agents present that the cooling effect caused by the choline esterase in vivo (Ache) activity decreased leaving acetylcholine (Ach) was increased, since the process of temperature regulation in 【Ach. In addition, the cholinergic system is also involved in the regulation of cardiovascular activity 【. 4J. Therefore, the experimental observation of the organic phosphorus pesticide chlorpyrifos (CHIP) and the cooling effect caused by the relationship between blood pressure. 1 Materials and methods 1.1 Experimental animals and reagents with 3 months old Long-Evans rats. CHIP chemical reagents for the U.S. Environmental Protection Administration Services products. 1.2 5% Shu animal surgery intraperitoneal injection of pentobarbital sodium anesthesia (50mg/kg) animals, in accordance with the conventional surgical method of abdominal skin and muscle incision, isolated aorta, the wireless remote sensor on the casing pressure test into the aorta, with a strong glue to fix, and the sensor fixed on the abdominal muscles,
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, and then sutured incision, the last intramuscular injection of penicillin to prevent infection. After surgery animals were kept in individual cages in a standard plastic rat. Animal laboratory temperature was 22 ℃, light and dark all the time as 12h. 1.3 The method of measuring body temperature and blood pressure after surgery 10d experiment. Measured with a wireless remote temperature and blood pressure changes. The abdominal cavity wireless telemetry sensors built into the normal feeding rats the same standard cage, so that animals are free activities, food and water status,
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, and then put the receiver on-board telemetry instruments,
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, animals, Wireless remote sensors measured temperature and blood pressure after receiving the signal from the receiver board, the analog signal input into the computer observation, storage and data processing. 1.4 The steps were divided into experimental group and control group and ClIP group, 8 rats in each group to allow animals
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