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Wysłany: Nie 23:53, 06 Mar 2011
Temat postu: tory burch qxb uvn owxj dnl
Microtubule-associated protein -2 in the hippocampus of AD model rats and its significance in
a-ryculturedhippoeampalneuronswereexposedto50 ¨ mo1SNPfor24hafterpmtreatmentwithdifierentconcentrationsofSF (10 ~ 160 ~ mol/mlfor6h.ThenneuronalviabilitywastestedbyM1-I1assay.FluorescentstainingwithHochest33258wasusedtoanalyzeapoptosis.TheexpressionsofNF-KB1 '65. par-4mRNAandproteinweretestedbyRT.PCRandWesternblot.ResultsPretreatmentwithSF (1O ~ 160tLmol/m1) for6hincreasedtIlesurvivalrateofneurons.SFpreventedtIleneuronalnucleifromshrinkage.condensationandcleavagein.ducedbySNPSFalsodecreasedtheexpressionsofmRNAandproteinofNF.KBP65andpar- 4.CondusionsSFcanpreventtheculturedhippocampalnell/ORSagainstSNPneurotoxicity.ThemechanismofprotectionislikelyrelatedtodecreasingthelevelofNF.KB1 '65andpar-4. 【Keywords] Sodiumferulate (SF); Sodiumnitroprusside (SNP); Culturedhippocampalneurons; Apoptosis; NF-KBP65; par-4 study found that free radical damage is caused by Alzheimer's disease (AD) is one of the important reasons,
tory burch
, NO is closely related with the onset AD and NO generation, and antagonistic pharmacological effects. Recent studies have found, sF can prevent a rat model of multiple sclerosis, oligodendrocyte death,
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, inhibition of neuritic response. sF AS induced by inhibiting the inflammatory response and p38MAPK activity in the hippocampus, reducing large hippocampal pyramidal neuronal damage and improve learning and memory in rats】. ChoH study found that long-term application sF, the suppression of cerebral intraventricular administration of the Fund Project: Medical scientific and technological project of Henan Province (200703002) 1 Experiment Centre Correspondence: Single Kit (1957 a), female, professor, master teacher, in tumor pathogenesis and biochemistry of aging. Author: Zhang wind (1972 a), female, lecturer, Master of Medicine,
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, Biochemistry in Aging. AI3-, 0 ~ l from the activation of astrocytes, decreased eNOS expression and protect brain function. but sF used in primary cultured hippocampal neurons as well as on primary cultured neuronal apoptosis not been reported. In this study, NO donor SNP induced primary cultured hippocampal neurons was observed sF SNP-induced apoptosis of neurons, and apoptosis related gene NF-KB1'65; par-4 expression, and to explore the possible neuroprotective effect sF mechanism. 1 Materials and methods 1.1 Reagents newborn (within 24h of birth) SD rats 64, female open, for the Experimental Animal Center of Henan Province, animal production license Zheng Hao as SCXK (Henan) 20o5-oool; DMEM, N nerve cells additives, horse serum, glutamine were purchased from Gibco Company; FBS Hangzhou Evergreen products; SNP, trypsin, cytarabine,
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, polylysine were Sigma products; mouse anti-rat NF-KB1'65 first antibody, rabbit anti-rat par-4 first antibody,
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, mouse anti-rat an Actin-8
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