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ncycgdxhl
Wysłany: Wto 12:26, 08 Mar 2011
Temat postu: Experimental animal model of femoral head necrosis
Experimental animal model of femoral head necrosis in the pathophysiology of Preparation
But generally can only copy of the pathology of early osteonecrosis, and the hormone sensitivity to animals there are individual differences, difficult to standardize the model of femoral head necrosis in the secondary replication, mainly through the destruction of or interference with the femoral head of the revascularization, such as WoodHouse reversed by cutting off the femoral neck Methods round ligament femoral head necrosis secondary ..., but because of animal bone regeneration and revascularization was higher than that of mankind, the success rate is not high,
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, and surgery on the hip joint function severely affected The ideal animal model should be simple and easy preparation programs,
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, the success rate can replicate in the short term model of the different stages of osteonecrosis. The present study, hydrochloric acid, sodium hydroxide injection of human femoral head necrosis model to replicate with the following features. (1) simple, high success rate; (2) joint function in animals has little effect; (3) hydrochloric acid and sodium hydroxide reaction is HC1 + NaOH = H2O + Naa, no systemic toxicity in animals; ( 4) copy the model of bone necrosis of trabecular bone necrosis, bone necrosis, bone dysfunction, and the characteristics of microcirculation is damaged, so the model can be observed in the dynamic evolution of femoral head necrosis in pathological experimental drug for the treatment of grave with a 3. 2,
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, the pathogenesis of osteonecrosis of femoral head necrosis in the pathology of primary mechanism,
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, Jones said: The end result of different causes of microcirculation damage, microcirculation is the primary SUI common pathological mechanism of femoral head necrosis, on the basis , the paper further considers that, whether primary or secondary osteonecrosis, the factors that determine the pathological development can be summarized in three aspects: ① etiology; ② osteoclast-mediated bone resorption and bone renewal rate effective the balance; ③ biomechanical effect. Eventually causes damage caused by micro-circulation, bone cells, bone marrow necrosis, bone marrow stromal cells into osteoblasts reduced ability of bone cells and attached to the trabecular surface osteoblasts have some role in barrier activity of osteoclasts l . When the femoral head necrosis of osteoclasts on trabecular bone loss of the protection of absorption increased trabecular bone absorption becomes so small and sparse on the other hand, because trabecular bone necrosis in the loss of mineral metabolism, and its mechanical strength fall, when the stress transfer, the trabecular bone necrosis of fracture easily absorbed at the same time, with the repair and restoration of blood supply to the state depends on the strength, trabecular bone surface of the functional state of membrane and bone marrow, bone marrow mass proliferation of cell division, necrosis occurs in the bone fibroblast-like mother cell-rich connective tissue, capillaries regeneration, bone lining cells and residual bone marrow stromal cells from the osteoblast differentiation of bone matrix synthesis the reconstruction of trabecular bone necrosis, usually in the normal and necrotic bone tissue area between the emergence of a repair, the area of bone formation and absorption are more active I4j, if the bone can not be absorbed by the new bone effective alternative,
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, will develop into a softening hide, when the mechanical load transfer in this area, the one hand, w. 11f Law stimulate bone reconstruction. On the other hand can also lead to fractures, causing collapse occurred L5j, therefore, bone uptake rate and the effective renewal rate of new bone to maintain a certain balance should be, and this relationship ultimately by the activity of osteoclasts, bone marrow stromal cells the ability of osteoblast differentiation and osteogenic cells in the functional status of the decision, in the femoral head necrosis in the pathological process of evolution has an important role.
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