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Wysłany: Czw 11:41, 24 Mar 2011
Temat postu: GHD glätteisen lzj haz oxla dfs
The radial artery puncture percutaneous coronary intervention in patients with wrist Nursing
Method is reliable,
GHD glätteisen
, simple, rapid, accurate, and can be used as the control Tianwangbuxin film quality. Key words Tianwangbuxin film tanshinone Ⅱ A high performance liquid chromatography (HPLC) Determinationoftanshinone Ⅱ AinTianwangbuxintabletbyRP-HPLCHUTaideZHENGXiaopingKUANGGang (ChongqingInstituteforDrugControl. Chongqing400012) [Abstract] ObjectiveToestablishaRP-HPLCmethodtodeterminethecontentoftanshinone Ⅱ AinTianwangbuxintable. MethodsmethanoI-water (75:25) wasusedasthemobilephase; thedetectionwavelengthwas270nm. ResultsTheregresseequationwasY = 6869X a 12.753 (r A 0.9999). TheIinearrangeoftanshinone Ⅱ Awas0.013536g a 0.27672gg. Theaveragere-coverywas99.33withRSD1.6% (n a 6). ConlusionsItcanbeusedforthequalitycontrolofTianwangbuxintable. [Keywords] Tianwangbuxintable; tanshinone Ⅱ A; RP-HPLC Tianwangbuxin film by the Salvia, Angelica, Shichangpu, Codonopsis, Poria, Schisandra, Ophiopogon japonicus, aspartame, to yellow, Scrophulariaceae, Polygalaceae (system), Semen,
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, Bai Zi Ren, Campanulaceae, licorice, cinnabar form, can Yin and blood, make up the heart and soothe the nerves. Salvia is Fangzhong Jun drugs, tanshinone Ⅱ A is the main active ingredient of acute and chronic neurodegenerative diseases have shown good results, there are anti-ischemic effect 【1], so the establishment of high performance liquid chromatography (RP- HPLC) determination of the content of tanshinone Ⅱ A. An instrument and reagent HP a 1100 HPLC, quaternary gradient pump, UV detector, HP ChemStation, ultrasonic cleaning, Kunshan Ultrasonic Instrument Co., Ltd., KQ a 500B type); methanol HPLC grade water Ultra-pure water. Tanshinone Ⅱ A reference substance (China Pharmaceutical and Biological Products, with determination, batch number 110766-200214), Tianwangbuxin film (Chongqing Tianjiao Pharmaceutical Co., Ltd., batch number: 060301,060304,060307). 2 Methods and Results 2.1 Chromatographic conditions Column: phenomenexCLC-ODS (5gm, 200mm × 4.6mm),
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, a mobile phase of methanol water (75:25), flow rate lml / min, detection wavelength 270nm, column temperature to 3O ℃. Number of theoretical plates tanshinone Ⅱ A peak should not be less than 363000. 2.2 2.2.1 Preparation of the solution preparation to take control solution tanshinone Ⅱ A reference standard, accurately weighed, add methanol produced per lml contrast with 16gg product solution. 2.2.2 Preparation of the test solution under the weight difference between taking this product, small study, accurately weighed about 4g,
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, home brown bottle, precision by adding methanol 25m1,
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, Mesa, said that given the weight, ultrasound treatment 4O minutes , let cool, dense plugs, then said that given the weight of methanol to complement the weight loss, shaking, filtration, the filtrate obtained, that is, too. 2.2.3 Preparation of the negative control solution prepared by non-prescription medicine Danshen samples, negative control solution with the legal system, that is, too. 2.3 Feasibility study to take standard solutions, the negative control solution, test solution, in the above chromatographic conditions, sample lOrd, the results shown in Figure 1. Shows a negative control for determination of tanshinone Ⅱ A no interference. 2.4 Investigation of precision linear drawing standard solutions, each sample 1,2,4,8,12,16,2 ottl, tanshinone Ⅱ A determination of the peak area to sample volume as abscissa and peak area for the vertical coordinates, the regression equation:: Y a 6869X a 12.753 (r = 0.9999). Linear range: 0.013536 ~ 0.27672gg.
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