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Wysłany: Sob 13:38, 23 Kwi 2011
Temat postu: Non- alcoholic fatty liver disease in peripheral b
Non-alcoholic fatty liver disease in peripheral blood T cells and subsets of and significance of
Abstract Objective To investigate the non-alcoholic fatty liver and peripheral T cell subsets and their significance. Methods A2PAAP bridging ELISA assay in peripheral blood of patients with the normal group and the T lymphocyte subsets CD3, CD4, CD8 and calculate the percentage of CD4, CD8 ratio, and compare the differences. Results of non-alcoholic fatty liver patients CD3, CD4, CD8 less than normal (P Key words non-alcoholic fatty liver disease in peripheral blood T cells in autoimmune test B, hepatitis C virus liver T lymphocyte subsets in peripheral blood of patients have been reported in the literature, see [1,2]. However, there has not been determined non-alcoholic fatty liver T cells in peripheral blood of patients reported. Is well known that the level of peripheral blood T lymphocyte subsets in the human body is a reflection of an important indicator of environmental stability. To this end, we have non-alcoholic fatty liver in patients with peripheral blood T cells and their subsets, the results reported below. 1 Materials and Methods 1.1 General information of non-alcoholic fatty liver patients (patient group) 51 cases were clinically diagnosed patients, including signs, blood biochemical liver function test ultrasound. Normal group, 49 patients in our hospital are qualified medical prevention and health care of healthy people, heart, lung,
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, liver, kidney and other vital organs without disease, liver and renal function was normal. The remaining two groups in terms of age, no significant difference. 1.2 Determination of conventional intravenous blood sampling, heparin (25u/ml), blood when the action accurately and rapidly, to avoid air bubbles or clots. Preparation of mononuclear cells and the day of production, the same day or next day as required fixed, stained, microscopic examination. Bridged by ELISA assay A2PAAP T lymphocyte subsets CD3, CD4, CD8 and calculate the percentage of CD4, CD8 ratio. Operating in strict accordance with instructions. 2 results of two sets of peripheral blood T lymphocytes determination results in Table 1. Peripheral blood T lymphocytes in the two groups Table 1 Determination results Note: The t test, compared with the normal group, the value of patient group differences were statistically significant (P <0.01) 3 Discussion T lymphocytes are immune cells of the body, according to its function and the different surface markers, can be divided into several subgroups. Under normal circumstances, all in a state of dynamic equilibrium between subgroups, they interact with each other constraints, to participate in the immune response. T lymphocyte subsets in the relative stability of the body play an important basis for normal immune function. Recent studies suggest that, in addition to CD8 cells can cause infection of the liver cell damage and apoptosis, but it also has a high CD4 cell cytotoxic activity, which can induce delayed hypersensitivity, induce apoptosis [3,4] . Therefore, to ensure that normal T lymphocytes in the immune physiological function between the number of subgroups, the relative stability of the quality is very important. Results of this study: non-alcoholic fatty liver patients CD3, CD4, CD8 less than normal (P <0.01), CD4/CD8 ratio lower than normal (P <0.01). This result confirms: Non-alcoholic fatty liver patients with T cell subsets and function of the number of abnormal, T cell subsets were significantly disordered, dynamic balance between the various subgroups was broken, so the body appears immune dysfunction , a series of pathological changes, as autoimmune diseases. This may be due to the body and T cell subsets and cytokines in the disorder, leading to a non-alcoholic fatty liver disease in patients with recurrent persistent, difficult to cure. In short, non-alcoholic fatty liver in patients with peripheral T lymphocyte subsets, for understanding the patient's immune status and the pathogenesis of all have some clinical value. Close attention to the non-alcoholic fatty liver in patients with T lymphocyte subsets in the dynamic changes, and timely provide the immune regulation therapy in improving non-alcoholic fatty liver patients with liver cell injury, avoiding the development of liver fibrosis in patients with to much sense needs further study. 【Reference】 January 1 Liu & HEALTH. Hepatitis B cells and peripheral blood T cells and subsets of. Huaihai Medicine, 2001,19 (3) :186-187 . 2 David Jiang, Liao Changgui. acute viral hepatitis and T lymphocyte subsets in peripheral blood analysis. Sichuan Institute of Health Management, 2005,3 (24) :10-11. 3JungMC, PapeGR.ImmunologyofhepatitisBinfection.LancetInfectDis, 2002,2:431. 4 Shao Lihua, Mali Xian, Lu Hui. liver tissue in patients with chronic hepatitis B HBVDNA quantitative and inflammation and apoptosis relationship. Clinical Laboratory Science, 2004,22 (2): 1171.
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