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Light on the chicken liver, pancreatic enzyme histochemistry

85p (|) l1 ~ 135 One Man Village. Zeng make a pig of Animal Science and green. 1985I (1) le ~ 237 pay attention Ling. Si-Yi University. 1987 | 18 (2). 208 ~ 2l3Influence0fLightingonEnzymeHistoehemistryofLiverandPancreasinChickenHuan $ Jianzhenet (DepartmentofHistologyandEmbryology, Veterinaryc. UeBeofPLA) ANmantSeven-tWOStarbrobroilerchicksof14-day-oldweredividedintoexperimentalandcontrolgroups. Theexperimentalchickswert ~ illuminatedwithincandescentlamps (15lx, 1Dl3D) andthecontrolchickswj twenty Inaturaltight. Comparingwiththecontrolchicks. thebodyweight. ofexperimentalonetlissomewhatheavierbutconversionefficiencyslightlylower. andtheactivitiesofATPase, acidphospllat chu, lactatedehydrogenmminliverandpanct ~ sin-creaseandthatofsucolnatedehydrogenaseinthepancrea ~ ealsorises, Theresultsuggeststhattheenergymetabolism, substancedig ~ tiOfl, anaerobicglycolysisoffiverandpancreaticoell,ugg italia, andaerobicmetabolismofpancreaticcellalestrengthendafterliglIng. (Xherwi ~, theactiviviesofsucolnatedehydrogenaseinfiverandalkalinephosphatase ,5-nucleotida ~. Niu liver rabies virus in the ordinary course of cell culture in general no obvious cell lesions. virulence titration more difficult. the domestic application of most of London is still the classic laboratory titration of mouse brain. This law continued a long cycle (2 to 3 weeks) operational problems, not by squeezing. We used methyl cellulose (Mc) l was established to replace ordinary agar plaque method for rabies virus rhyme. In 24 holes or l6-well culture plate, the deer adapted strains of rabies virus 8202BHKn do to a different gradient. synchronous inoculated BHKzI cell suspension culture 24h, suck nutrient solution, added with MC, covering nutrition, and then cultured 3 ~ 4d, disposable suction cover, fixed with crystal violet staining of formalin, you can count plaques. of deer virus BHK: t13 on behalf of the adapted virus titration by repeated 6 times,ugg italia, and its toxicity titer (3.50 �� 1.29) �� 10PFO/mL (the average number of persons plaque Cool

. This method sustained period is short,oakley brillen, 3 ~ 4d can be used to determine the results of t within the eye and the specific and sensitive t reproducible, simple, economic, easy to spread,converse all star, to solve the rabies virus without obvious cytopathic effect due to the difficult problem of titration, a certain application value, both for the virus clones, but also for virus titration, and neutralizing antibody detection. (Veterinary Institute of Military Veterinary Yuejun Ming University,ugg boots italia, World Wide Yin Zhen Hou)