 Wysłany: Nie 23:53, 13 Mar 2011 Temat postu: The methylphenidate transdermal properties _3149 |
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| Methylphenidate transdermal characteristics of the
A + A + A + A +··+-+ A + A + A + - + A + A + A + A + A + A + A + A + A + A + A + + a + a + ten A + a + ten + One + - + A + A +. + A + + + A +. +. +. +. EpitopeDNAvaccinesagainsttuberculosis: spacersandubiquitinmodulatescellularimmuneresponseselicitedbyepitopeDNAvaccineWangQM, SunSH, HuZI, ZhouFJ, YinM, XiaoCJ,tory burch outlet, ZhangJC (DepartmentofMedicalGenetics,keen shoes sale, SecondMilitarvMedicalUniversity, Shanghai200433, China) [ABSTRACT] Cell-mediatedimmuneresponsesarecrucialintheprotectionagainsttuberculosis. InthisstUdy,mbt scarpe outlet, wecon-structedepitopeDNAvaccines (p3 a M38) encodingcytotoxicTlymphocyte (CTI) epitopesofMPT64and38kDaproteinsofMycobacteriumtuberculosis. Inordertoobservetheinfluenceofspacersequence (AIa-AIa-Tyr) orubiquitin (UbGR) ontheefficacyofthetwoCTIepitopes, wealsoconstructedDNAvaccines, p3 a M-S (spacer) for a 38,tory burch reva, p3 a Ub (UbGR) an M-S a 38andD3-Ub-M a 38. TheimmuneresponseselicitedbythefourDNAvaccinesweretestedinC57BL / 6 (H a 2) mice. ThecytotoxicitvofTcellswasdetectedbyI. DH-releasemethodandbyenzyme-linkedimmunospotassayforepitope-specificcellssecretinginterfer-on-gamma. TheresultsshowedthatDNAimmunizationwithp3 a M a 38vaccinecouldinduceepitope-specificCD8 + CTIre-sponseandthatthespacersequence (AAY) onlyenhancedMepitopepresentation. Theprotein-targetingsequence (UbGR) enhancedtheimmunogenicityofthetWOepitopes. ThefindingthatdefinedspacersequencesatC-terminusandprotein-targetingdegradationmodulatedtheimmuneresponseofepitopestringDNAvaccineswillbeofimportanceforthefurtherdeveloDmentofmulti-epitopeDNAvaccinesagainsttuberculosis. [ScandJImrnunol,UGG boots Italia, 2004,60 (3) :219-225] ¨ mouth one by one ~ ~ ~ a ~ one hundred and eleven one hundred and eleven one by one ~ ~ a ~ ~ one thousand one hundred eleven one hundred and eleven of a ~ ~ ~ one by one A ~ 111111111 ~ a |
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