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| Cancer gene therapy delivery system New Development
The form of liposome applications. However, the recent cationic lipid emulsion as a non-viral gene vectors also studied. Cationic lipid is one or two fatty acids (acyl), or alkyl side chain, a connection key and a hydrophilic amino composition of amphiphilic molecules. Liposome / DNA complexes known as lipid complex (1ipoplex). Freeze etch electron microscopy and x-ray diffraction experiments show that,ugg boots italia, DNA is the number of liposome particles caught in the middle. Because of poor stability, lipid compounds are usually used immediately after preparation. Complex lipid particle size, zeta potential, DNA /)] ~ quality ratio, and many other physical factors in the body affect complex formation, stability and transfection efficiency. Structure-activity relationship of cationic lipids mainly from practical experience. Inserted in a neutral lipid (total lipids) increased lipid complexes in vivo transfection capability. The most commonly used cholesterol and total lipid was the second oil acyl phosphatidylethanolamine (DOPE), the former is more effective in the body, which can enhance the in vitro transfection. Protamine is an arginine-rich peptides can form complexes with cationic lipids with negatively charged DNA before condensation. The role of liposome and DNA condensation can form a tight lipid rearrangement and the liposome / DNA complexes (LPD). Size distribution of LPD was 100 ~ 250nIn, for traditional lipid complex 1 / 5 ~ 1 / 3. LPD 4 ~ C or freeze-dried after 4 months at room temperature is stable and does not affect the transfer capability. The results show that in vivo, LPD-mediated gene transfer effect is stronger than conventional liposomes, smaller particle size but also promote the endocytosis and prolong in vivo circulating half-life. Intravenous injection of animal models of cancer tumor suppressor gene Rb, or carrying E1A of DOTAP: Chol-LPD, can induce apoptosis, reduce tumor size and prolonged survival time. Recently, through the interaction between the LPD charge to the liver targeting ligand-free saliva fetuin (asialofetuin) package, HepG2 hepatoma cells significantly increased the uptake of DNA on the package. Dc-Chol/DOPE LPD composed of 2 cases of cerebral cortex used for treatment of children within the ASPA gene in Canavan disease, has shown clinical effects. Cancer Gene Therapy 3 Conclusion excellent gene delivery agents must be able to package and protect the nucleic acid to avoid degradation of endosomes, and specifically targeting the tumor. Treatment with some breakthrough is expected that the market potential and billions of dollars excitation, the field of gene therapy will be quickly developed and strengthened. Progress in the treatment of siRNA compiled Liu Liping (Chinese PLA General Hospital, Institute of Cardiac Surgery, Beijing lO (I) 27) Abstract: The role of small molecules with interference RNA (siRNA) gene allows the expression of mammalian somatic cells stop, so , if you know the cause of a disease gene mRNA sequence,ghd italia, you can use to block the expression of siRNA. Currently, siRNA's into two main methods, namely, exogenous and endogenous means way. Experiments show that,mulberry bags, siRNA can make a variety of gene silencing within the cell, so in addition for the analysis of gene function, to determine the effectiveness of the target object, but it is also in gene therapy has great value. Key words: small-molecule RNA; gene; treatment CLC: Q527 Document code: A Article ID :1001-0971 (2004) 05-0269-04RNA interference (RNAi) was first in the Caenorhabditis elegans (Caenothztbd / t / selegans) a phenomenon found in the body,herve leger sale, later found to exist in plants, the same phenomenon,adidas scarpe, calling it post-transcriptional gene silencing (IX) st-transcriptionalgenesilencing, frI'Gs), is now considered a monitoring system, are mainly used for blocking the production of potentially harmful or RNA expression. RNAi and FIGS start by double-stranded RNA (double-strandedRNA, dsRNA) Received: 20O4436-O7 caused. Of C. elegans, the function of dsRNA quantitative analysis showed that only part of each molecule in a cell to activate the role of this particular silence. Although the distractor response pathway activation, by long-chain dsRNA (38 bp or more) RNAi in mammalian cells is difficult, but studies have shown that small molecule RNA (siRNA, containing 21 nucleotides) can be successfully block the expression of RNA. Subsequently many vivo and in vitro experiments show that the siRNA has potential therapeutic effect. In theory, mRNA |
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