 Wysłany: Sob 12:24, 19 Mar 2011 Temat postu: HPLC determination of Scutellaria Ning ointment an |
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| HPLC determination of ointment baicalensis Ning baicalin and hydrochloric acid content of Dyclonine
Key words: HPLC thymol SA CLC: R927.2 Document code: A Article ID :1672-7738 (2007) 10-0599-03SimultaneousdeterminationoftwocomponentsincomplexthymoleardropsbyHPLCHEYi-feng, LIUYi-zhao (theFirstPeople ' sHospitalofChangdeCity, Changde415003) ABSTRACT: OBJECTIVEToestablishamethodforthedeterminationofthym'olandsalicylicacidincomplexthymoleardrops. METHODSThecolumnwasshim-packVP-ODS (4.6 × 150mm, 5um), themobilephaseconsistedofmethanol - wa-ter-phosphoricacid (60:40:0.3), thedetectionwavelengthwas220nm, thecolumntemperaturewas30 RESULTSTherewasagoodlinearrelationshipwhenthecontentofsalicylicacidandthymolwasintherangeof0.12 ~ 0.28mg · m = a,mbt shoes utlet, and0.06t0.14mg · mL-respectively. Theaveragerecoveriesofsalicylicacidandthymolwere101.2 (RSD = 0.57) and100.1 (RSD = 0.64), respectively. CONCLUSIONThemethodCanbeappliedtOthequalitycontrolofcomplexthymoleardrops. KEYWORDS: HPLC; thymol; salicylicacid ear drops containing compound thymol thymol, salicylic acid and other ingredients. For the treatment of ringworm better ear. In order to control its quality, relevant literature _l ~,UGG boots Italia, this study established a HPLC method, simultaneous determination of 2 ingredients preparation method can be used for the preparation of quality control. An instrument and reagent a Shimadzu LC 10A HPLC, Class ~ vp6.12 chromatography workstation,tory burch, thymol, salicylic acid reference substance (China Pharmaceutical and Biological Products), compound thymol eardrops ( hospital preparation room production, batch number: 070323,070518,070727), methanol (HPLC grade), phosphoric acid (AR), water (twice distilled). 2 Methods and Results 2.1 solution preparation 2.1.1 Preparation of reference substance stock solution; accurately weighed thymol 20.0mg, salicylic acid 40.0mg, with the home 100mL flask, add methanol and dissolved diluted to the mark, shake, reserve liquid as a reference substance use. 2.1.2 Preparation of test solution: precise amount of the test 5.0mL, home 100mL volumetric flask, diluted with methanol to the mark,MBTシューズ, shake, then take this solution 5.0mL to 25mL volumetric flask, add methanol and diluted to the scale, shake spare 2.1.3 Preparation of blank solution; except thymol and salicylic acid, the amount of materials weighed prescription, preparation of the blank sample solution. Click 2.2 Chromatographic conditions and system suitability test column: Shim-packVP-ODS (4.6 × 150mm, 5m), mobile phase: methanol and water a phosphoric acid (60:40; 0.3), flow rate: 0. 8mL · min-, detection wavelength; 220am, the injection volume: lC'L. In this chromatographic conditions,ghd piastre, the measured retention time of salicylic acid and thymol were 5.3min and 17.6min, thymol theoretical plate number 10600. Ih'Jute $ A: blank matrix diagram |
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