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一株具有抗HBc及抗HBs双重反应性的抗HBV单克隆抗体细胞…
1张定丑乙型肝炎病毒见.妊定凤主编己堑肝兜盯发病机理与临床.重庆:重庆出版社.1992.1—232StandriugDN.MolecularanalysisofhepatitisBvirus.ProgLiverDist198616:1913祁进龙.部家育单克隆抗体及其临床应用f讲座)国外医学临床生物化学与检验分册.1988:9(3)244李方和,杨末亮,喻檀群.一种简便快速的YkAb筛选方法及其在单克隆抗体培养上清鉴定中的应用.单克隆抗体通讯,1993;9t2):675王洪簿综述.单克隆抗体免疫交叉反应现象及其原围探讨.单克隆抗体通讯,1992,ralph lauren outlet;8(1)496FernandazPA,TernynchT.vrShn—munochemiealstud[es(farnurune!JolyreacnveIgG2bautoantibodyw;thRFaetivtyMolecu—larImmunology,Tory Burch outlet,989}26(65X9(L99311—09收稿)EstablishmentofaCellLinewithAnti-HBVMonoclonalAntibodywithTwo—foldImmunoreactivitiesagainstBothHBsAgandHBcAgLiFanghe,YangDongliang.YuZh[qunetalClinicalimmunologyResearchgnit.7~ngjlHospitat.TonMiMedical[fnlver.~.&y.~Vuha;,l430030AhstractInthepresentstudy,ahybridomaeeljline(E11)wasestablishedbyfusionnfmousemyelomacells(Sp2/0)withspleencellsfromBALB/emiceimmunizedwithttBVantigenpurifiedfromhumanplasmaandcontainingaplentyofHBVDaneparticles.ThemonoclonaIantibody(McAb)secretedbvE11eelIIinehastwofoldimmunoreactivitiesagainstbothHBsAgpolypeptides(HBeAg),mbt zapatos,HBVantigencontainingaplentyofDaneparti—des.HBVlightroundparticles(bothofthemcomefromhumanplasma)andHBsAgpositivehumanserumasproredbymultipIemethods.Thetiters0ftheMcAbascitesthatreactedwithabovefourHBVantigenswerebasicallysame。butitsimmunoreactivityagainstHBeAgwasstrongerthanthatagainstotherthreeantigens.ThespecificityoftheMcAbimmun0reactivitvwithabovementioaedHBVantigenshasbeenprovedbyblockingtestsandsubstitutiontests.Keywordsantibody,monoelonaI;hepatitisBvirus;antigenicspecificity;enzymelinkedimmunosorbentassay
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