asbryobvrz
ORANGE EKSTRAKLASA
Dołączył: 21 Lut 2011
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 Wysłany: Wto 14:03, 08 Mar 2011 |
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Antigen gene of Schistosoma japonicum 14-3-3 Cloning and sequencing
Fluke 14-3-3epsi1on subtype gene (Acces-sion: AF195529). Zhang and other irradiated cercariae with serum obtained from adult eDNA library was screened out of the Et of the schistosome 14-3-3 proteins. The protein contains 254 amino acids,[link widoczny dla zalogowanych], present in all stages of schistosome development. Preliminary studies have shown, 14-3-3-positive sera can be identified with Schistosoma mansoni and Schistosoma haematobium no cross reaction,[link widoczny dla zalogowanych], suggesting the development of 14-3-3 with the value of diagnostic antigen. The protective immune effect of further study 【3】. In this study,[link widoczny dla zalogowanych], from Schistosoma japonicum (mainland strain) cDNA was amplified in vitro gene encoding 14-3-3,[link widoczny dla zalogowanych], PcR product using TA cloning into pGEM-T vector. And double enzyme digestion and the recombinant plasmid as a template for PcR, be confirmed. Sequencing results showed that it has a 765bp complete ORF. Encoding 254 amino acids. In this study,[link widoczny dla zalogowanych], the cloned gene encoding 14-3-3 expression vector in eukaryotic Subcloning and expression of the encoded fusion protein and its characterization is under way. 4
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