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 Wysłany: Śro 8:32, 16 Mar 2011 |
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十大功劳属部分植物茎中生物碱的高效毛细管电泳法测定
i(BeOingUniversityofChineseMedicine，Beijing100029；121epartmentofChemistYy，PekingUniversity，BeQing100871)ABSTRACTAIM：TodeterminethecontentsofthreealkaloidsinsomeplantsofMahoniaandtoprovideaneffectivetoolforthequalityevaluationofMahoniafarnily．METHODS：Thecontentsofthreealkaloids．berberine，palmatineandjatrorrhizine，indifferentplantsweredeterminedhyusinghighperformancecapi[1aryelectrophoresis(HPCE)，withbrucineasinterna1standard．Theconditionswereasfollows：diodearraydetectorsetat265nm；runtime，8，0min；appliedvoltage，30kV；temperature，20℃．Thebackgroundelectrolytesystemselectedwas0．1mol·Lph~phatewithpH7．0．methanoI(2：1)．REsUL1：TheIinearrangesofberberine，pahnatine，andjatrorrhizinewere4．9-498．6·mLI1(7=0．9990)，5．0-504．9g·mL(7：0．9996)and5．1～505，8vg。mL(7=0．9984)，respectively．TherelativestandarddeviationsofpeakareaofthealkaIoidsrangedfrom1．03％to1．61％．TheaverslgerecoveriesofthealkaIoidswere96．00％～101．66％，100．15％～102．97％and96．68％～102．44％，respectively．QuantitativedeterminationofthealkaloidsinthedriedstemofdifferentplantsofMahoniawasperformedbythismethod．Theresultsshowedthatthecontentsofthethreealkaloidsvariedgreatlywithspecies，placeoforiginandcollectingtime．CONCLUSION：ThecontentsofthealkaloidsinalItheplantsofMahoniaanalyzedinthispaperwereveryhigh，s。theyhavethepotentialofbeingnewpharmaceuticalresources．HFCEtechniqueprovidesasimple，rapid，andeffectivetooIforthequalitycontroIofherhalmedicine．KEYWORDSMahonia；highperformancecapillaryelectrophoresis(HPCE)；herherine；palmatine；jatrorrhizine
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