ncycgdxhl
ORANGE EKSTRAKLASA
Dołączył: 13 Gru 2010
Posty: 502
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Wysłany: Nie 20:22, 20 Mar 2011 |
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pEGFP-N1-α-synuclein eukaryotic expression vector
-Synu. clein by restriction enzyme digestion , confirmed that the size of fragments in line with expectations ; confirmed by sequencing twenty synuclein pEGFP-N1 vector was constructed successfully. The pEGFP-N1 vector with the report of twenty synuclein EG-F'P,[link widoczny dla zalogowanych], transfected cells, fluorescence microscopy can be detected in green fluorescence ; FLAG as a label,[link widoczny dla zalogowanych], provided us with a method of detecting the target gene ; In addition, the carrier also contains a selection gene neo,[link widoczny dla zalogowanych], which can take advantage of neo G418 resistance gene to integrate with the carrier screening of eukaryotic cells. This study DEGFP. Twenty sy-nuclein N1 fusion gene expression vector for the future of a-synuclein in the dopaminergic nerve cell degeneration and necrosis of the role of a. synuclein in Parkinson 's disease patients have gathered in vivo role of LB basis . 【
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