Determination of serum calcium and phosphorus lipa

ncycgdxhl · Wysłany: Pią 8:40, 18 Mar 2011

Determination of serum calcium and phosphorus lipase D

However, these methods online, sensitivity. Repeatability, the stability of the color reagent segment,[link widoczny dla zalogowanych], protein interference and so can not be both serum calcium and phosphorus Based on the determination of lipase D analysis of the results,[link widoczny dla zalogowanych], to explore the feasibility of Method Laboratory should Hj: Test Principles II phospholipase Rubidium acid-choline + HzO phosphatidic acid choline choline + II +202 + mo Tun Mexico betaine +2 HzO2HzO +4 l of a 4-amino antipyrine ammonia a A + EMSE (N an ethyl one N. (3 a tolyl) a N, a] --- a acyl ethylenediamine) I (3.OkU/LP1_n, 30kU/LCOI1.40kU/IPOD, 25mmol/LEMSE,[link widoczny dla zalogowanych], 2Og / L bovine serum albumin .100mmol/LHEPES s solution, pH75). Reagent Ⅱ (3Ommd / L two phosphatidic acid choline, 6.4ram & eL4AA.1OOn ~ nol / LHEPKS s solution. PH75); Hitachi 7】 5U automatic biochemical analyzer only. Meter (Shanghai Third Analytical Instruments,): 2.2 specimens and medical personnel dealing with printed instructions from hospital patients. No related diseases by physical examination. Pumping blood (no anticoagulant) 2.0ral, 37 ℃ water bath】 h. Centrifuged, the supernatant determination to spare 6 【2.3) samples was with o-cresol phthalein complex method using (o-c-reso] Ⅱ haleinc Ⅷ x, cec) i ~ l be. According to the results of high and low, were divided into low-value group (L group),[link widoczny dla zalogowanych], the value of the group (M group) and high-value group (H group). 20 cases each. Serum was prepared by mixing, 20 continuous determination to do the within analysis. Measured in each group pooled serum with 10d. Mean daily statistics, asked to do batch analysis. In serum 10. Add reagents l240, reagents Ⅱ 6 【】, 37 ': 12 and incubated for 5min, 505/546tm ~ l be: 3 outcomes 3. 】 Precision of the right of Table Henan Henan County public hospital treatment and medical tests in 32 other drug information results in calcium ion concentration 2.27ttm ~ I / [serum were added to O. 25,0.50,0.75, I. 00,[link widoczny dla zalogowanych], I. 25 and 15 @ ~ mob'L calcium standard solution, a 92.0% recovery of prison 108O%; The linear range 0 ~ 4nur ~ I / I Table I Determination of serum calcium and phosphorus lipase D analysis of the results of obituary bit: KU / 14 to discuss mainly of calcium in serum ionized calcium, combined with the form of two kinds of calcium, about 50% of each present, most of the laboratory determination of total calcium only. Determination of total calcium in the blood are atomic absorption Shuguang Guang degree method, dyes Results bench method and titration method. Although the advantages of the method is prominent, but also have enough. Such as the CPC method vulnerable to MdF interference. Color depth and p | I related factors can cause friends Die negative deviation t; as though ho A simple complexometric titration. M interference free, easy to spread. However, Qi j indicates calcium red unstable in strong alkaline solution. Human error and determine the larger end: Ⅱ n clear determination of calcium and phosphorus lipase D; to interference from these factors, and the wide linear range, high sensitivity, reagent stability, especially for large double-reagent or biochemical automatic analyzer reagents . The method by hemolytic and anticoagulant effects are more evident. Phan blood, red blood cells in the release of organic phosphorus. Phospholipase D and then be broken down into inorganic phosphorus; in a variety of anticoagulants in. More stable calcium heparin, and EDTA or calcium citrate anticoagulation was significantly lower.