Epimedium Shengbaiyin preparation with astragalus

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Epimedium Shengbaiyin preparation with astragalus and Identification of medlar

Abstract Objective: Shengbaiyin of epimedium, astragalus, medlar for TLC identification. Methods: TLC, on the preparation of epimedium, astragalus, medlar, qualitative identification. Results: The TLC separation of the results to get a clear, simple, reliable and easy. Conclusion: drinking to enhance living standards of white provides a reliable basis for control of a white as the quality control of drinking. Key words Shengbaiyin; epimedium; Astragalus; medlar; identification papers from China Union finishing. Shengbaiyin by the epimedium, astragalus, medlar, artillery Shan Jia, human blood grass, buckwheat, composed of seven herbs and other second taste. Consolidate with Qi, nourishing fluid role. Hospital preparation room by the production of the main clinical Shengbaiyin for vertigo, Shenpi fatigue, anorexia, blood weak,[link widoczny dla zalogowanych], leukopenia. In order to effectively control the product quality, the author of its main drug epimedium, astragalus, medlar were TLC, the results of spot obvious, and reproducible. 1 instrument and reagent silica gel G, silica gel H (Qingdao Marine Chemical Factory), chromatography using neutral aluminum oxide (Lu are Shanghai Chemical Reagent Factory); medlar reference drug, Astragaloside icariin standard, and reference substance (all purchased from Pharmaceutical and Biological Products); reagents were of analytical grade; Shengbaiyin (hospital preparation room provided, approved No: 041001,050601,061001). UV analysis of black box (model YOKOZX), ultrasonic cleaning machine (model: KQ50B); electric oven (Model: GZXDH), digital temperature water bath (model HH2). 2 TLC 2.1 Epimedium experimental methods 2.1.1 Preparation of the test solution to take a white drink 5 ml, evaporated on a water bath, add ethanol 10 ml, ultrasonic extraction of 20 min, filtration, the filtrate evaporated and the residue add 1 ml ethanol dissolve, as the test solution. 2.1.2 Preparation of standard solutions of icariin take icariin made reference substance in methanol to 0.1 mg / ml solution, as the reference solution. 2.1.3 lack of negative control solution prepared to take Epimedium Shengbaiyin 5 ml, according to the preparation of the test solution, made negative reference solution. 2.1.4 TLC [1] to take the test fluid, control fluid icariin, negative control solution of the 10 μl, respectively, at the same point as the viscosity of sodium carboxymethyl cellulose mixture of silica gel H thin layer plate with ethyl acetate - methyl ethyl ketone - formic acid - water (10:1:1:1) as the agent, started out, drying, home UV light (365 nm), under review . Test products for chromatography, chromatography with reference substance corresponding position, was the same dark red spots; sprayed with aluminum chloride test solution, heated at 105 ℃ for 2 min ~ 5 min, UV lamp set (365 nm) Under the View, which was the same orange-red to yellow fluorescent spots. Negative control solution is no such spots, the results shown in Figure 1 (3. Sample batch number 041001; 4. Sample batch 050601; 5. Sample batch number 061001). 2.2 Astragalus experiments 2.2.1 Preparation of test solution taken Shengbaiyin 30 ml, evaporated on a water bath with 30 ml n-butanol extraction of 30 min ultrasonic , filtration, the filtrate added to a neutral alumina column (100 ~ 120 mesh, 5 g, diameter 10 mm ~ 15 mm), and with 100 ml 40% methanol elution, evaporated and the residue 30 ml of water allows the dissolved water saturated butanol shake extraction 2 times, 20 ml / second, combined n-butanol solution; water washing 2 times, 20 ml / second; discard the water liquid, butanol liquid evaporated and the residue add 0.5 ml of methanol to dissolved, as the test solution.