Modern Biotechnology in Forest Tree Genetic Breedi

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Modern Biotechnology in Forest Tree Genetic Breeding

Resistance genes in many plant breeding programs and genetic map of the important goals. DNA markers can be used with the resistance gene on linkage analysis of genomic regions to identify specific DN broken chromosomes, identifying molecular markers linked to resistance genes, and thus determine its position on the chromosome, as a token based selection and separation resistance gene basis. Michelmore et al reported the separation of groups of gene clustering methods for rapid expansion of a single location, the method and RAKP, AFLP and other techniques can greatly improve with the combination of linkage of the marker gene screening efficiency. Quality traits of disease resistance in forest trees, mainly on Poplar rust, pine blister rust, black spot of elm genetic research more, these patients have been found tightly linked with the marker gene,[link widoczny dla zalogowanych], and were localized in the linkage map, the other Using molecular markers to find new varieties of the South against Yang's material and insect pest suppression phase linked marker genes for insect resistance of poplar provide a basis for the feasibility of breeding. 3.3 Species identification and mapping in recent years, DNA fingerprinting, molecular markers are widely used in the identification of tree genotypes, embodied in the taxonomic tree, trees,[link widoczny dla zalogowanych], phylogenetic relationships, tree biology and genetic fingerprint mapping and so on. The emergence of molecular markers for the taxonomy of forestry workers with basic genetics will provide an opportunity for bonding. Methods of modern molecular taxonomic classification method has been replaced and applied to characterize taxonomic study of trees, the main techniques include: RAPD (random amplified polymorphic DNA), on the mtDNA of RnP (restriction fragment length polymorphism sex) and DNA sequencing of partial gene (such as: ITS, 18SrDNA,[link widoczny dla zalogowanych], 28SrDNA rapid evolution in the area and the gene encoding the protein). Yang is China, and other species of larch were asked Classification and relationship of molecular level studies, phylogeny, molecular evolution research and breeding strategy for the resources. 32 * 4 tissue culture plant tissue culture in agriculture and forestry are mainly used in the rapid clonal propagation of plants and the production of virus-free seedlings, in addition to it, and cultivate new varieties, germplasm preservation and artificial seed fields to the close relationship between . Of tissue culture founded on the 3o years, 7O flourish in this area since, 8O's and virus-free seed propagation of plants has been large-scale application. According to Luo Shiwei (1988) statistics, 28 families and 99 kinds of woody plants have been obtained clone, of which 74 species are generated through the embryoid way Regeneration. At the same time there are 26 species of pollen by anther culture plants. Such as: Department of the origin of Eucalyptus in Australia, has more than 200 eucalyptus species introduced into the country, and become the main southern region of timber trees, the Shanghai Botanical Garden introduction of Eucalyptus shoots from the callus induced complete plants, Qinzhou Forestry Research Institute of plus trees from the hybrid seeds or aseptic callus bred 1O test tube embryo cell lines. Conducted in China since the 70's that in vitro culture of poplar, initially focused on anther culture. Northeast Forestry University, has induced simonii (P.simonii), Xiaoqing Yang (P.pseudo-simonii) 10 types of poplar pollen plants; Beijing Institute of Botany, with Populus (P.tomentosa) dormant buds of plants induced a complete . Rare species of the genus Camellia, Yan Muqin to collect 21 kinds of tea,[link widoczny dla zalogowanych], of which 20 tissue culture success, they will Camellia (Camelliachrysantha), tea (C.oleifera), red tea (C.polyadonta) and tea (C.sinensis ) through the callus, somatic embryos, axillary bud and shoot fake beads produced a large number of cultured plantlets. Fir (Cunninghamialanceolata) is the most important in South China timber species. Subtropical Forestry Research Institute of Chinese Academy of Forestry and stem from the tip to form plant callus, plantlets cultivated close plants. Department of Paulownia witches original body type strain (MLO) caused the most serious diseases. Beijing Institute of Botany, with shoot tip culture and tetracycline treatment received more than 20 strains of disease-free seedling clones.