Clinical application of polymerase chain reaction

e707004304 · Wysłany: Pon 10:37, 28 Mar 2011

Clinical application of polymerase chain reaction

. In recent years, PCR for hepatitis C virus (HCV) research has increased, including the detection of HCV, HCV evaluation of interferon treatment on a response and a clear HCV chronic hepatitis after liver transplantation in the role. PCR using DNA enzyme immunoassay products can be detected as little 6.4pg RNA virus within the liver cells can be widely used in clinical practice. 4. Influenza virus: recent, with the influenza virus type-specific and subtype-specific oligonucleotide primers appeared, with the fast separation of viral RNA and cDNA synthesis methods, PCR amplification can be used to trace the virus and determine the type of influenza virus subtype, can be quickly and accurately identify new variants and their sources. Application in cancer genetics to determine changes in certain tumors has been of concern for cancer researchers. Sometimes, this change is not only disease markers, and Zhejiang Medical l6 1994 No. 6 can be used to evaluate treatment success. PCR can be used to detect tissue-specific genes have been identified in any cancer cell genetics PCR also detected the normal tumors, such as Philadelphia negative chronic myeloid leukemia by PCR can be found in dye-eup body translocation ', indicating that the PCR than the cytogenetic sensitive method of the present study, PCR and allele oligonucleotide hybridization of the end stations have been widely used in ra8 oncogene mutation detection in many malignant and benign tumors in the ras mutations have been found. in immunology, such as the HLA typing} ILA Ⅱ quickly identify the characteristics of antigen variation, detected a single base pair mutation. its speed, accuracy better than serological methods. Some scholars confirmed by PCR rheumatoid DW arthritis patients HLADR two subtypes (DW and DW) and disease susceptibility will help to clarify the pathogenesis of the disease Quantitative and so on. In summary, PCR is not only used for genetics,[link widoczny dla zalogowanych], virology, oncology and immunology, etc., can also be used to Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycobacterium tuberculosis, M. leprae, fungi and parasites for testing. At present, the difficulties of clinical application of PCR primers need to know, for a virus or bacteria and other pathogens can not be accurately quantified and the cost of the technology is more expensive, DNA amplification of highly contaminated. However, PCR technology more quickly than others are based, it can play in the clinical application of a key role. .