Centrifuged fat on biuret method of determination

e707004304 · Wysłany: Wto 22:55, 29 Mar 2011

Centrifuged fat on biuret method of determination of total protein

o. China Tropical Medicine 』Janury2006 6, 2006 No. 1 focus after identification of the mosquito eggs together until the eggs mature embryo (about 72h later), 18cm in diameter white enamel basin, Sheng 1500ml different concentrations of salt water around until the eggs hatch out, according to conventional methods and mixed-fed fish feed yeast (for the end of feed), the daily added a certain amount of suction and the corresponding concentration of salt water. 1.2.2 Methods of mosquito eggs counted into the different concentrations of salt water, the salt (NaC1) concentrations were 0% (water control), 0.1%, 0.3%, 0.5% 1.0% and 1.5%, daily observation hatch, grow, pupate situation. Observation of repeated measurements for each concentration of 1. 2 Results 2.1 Anopheles different concentrations of salt water in the hatching, growth and pupation (see table 1) in 0% of a salt concentration of 1.5%, 3 anthropophagus can hatch in water and low concentration of salt (0.5%), the hatching rate was no significant difference; mosquito child mortality increased with increasing salt concentration, 1,4-larvae grew slowly, death occurred in three larvae; of pupae rate increases as the salt concentration decreased, but the pupae into adult mosquitoes can feather, 1.0% salt illustrates the value of a chrysalis. Anopheles Table 13 in different concentrations of salt water in the hatching, growth and pupae of the situation (X10) Mino mosquito species egg counts (Continued from page 125) cleared the total protein content, the results show that the application of biuret in the determination of total protein, its wavelength on the determination of blood lipid levels and other wavelengths without interference in common, that is, can not be eliminated by dual blood lipid effects on the determination of total protein. 2.2 Mosquito Larvae in salt concentration and the growth cycle at room temperature (28 soil 2) ℃, the 3 anthropophagus in ≤ 0.5% after the hatching of brine concentration were pupae at the beginning 7d, the first of about 9d peak for the pupae, larvae of a minimum of 7d, the longest 15d; in ≥ 1% salt concentration, the start time and pupae pupae were later postponed the peak time 2d; concentration of 1.5% saline, even if they can some larvae hatched, the tiny child development, but l is the line of death. 1.5% salt concentration can not make three kinds of Anopheles mosquitoes from egg to, for the growth of Anopheles limit concentration. 3 Discussion Anopheles mosquito from egg to adult, breeding environment for a wide range of adaptability, but the different bodies of water on larval growth and development also has a great impact on their development needs in particular,[link widoczny dla zalogowanych], optimum temperature, pH, water cool , grow better in fresh water. Different regions of the ecological habits of Anopheles have some differences, both the latitude 42. Anopheles Liaoning, north or north latitude and 33. Anopheles south of Sichuan, or Anopheles island growth, in addition to good growth in fresh water, the salt water at low concentrations even in normal saline (0.85%) concentration, still normal hatching, growth and development and into mosquito pupae. The results showed that low concentrations of salt less effect on the eggs hatch, if it reaches a certain concentration, the larvae of Anopheles has great influence on the growth and development, the results show that: inland and islands Anopheles Anopheles Growth of Anopheles larvae in the water concentration limit should be 1.0% to 1.5% of the question, does not exceed 1.5%. This study shows that 3 anthropophagus have a certain ability to adapt to salinity, the growth of the island and domesticated Anopheles Anopheles island after the tolerance of salt water are basically the same, and the results can be seen from the its tolerance of salt water no less than the growth of inland Anopheles stronger, and thus prompt: Anopheles may be generally strong osmotic adjustment mechanisms and adaptability.