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yxfbbiedtj · Wysłany: Pon 3:58, 14 Mar 2011

Self-made cationic liposome-mediated gene transfer in vivo

Levels of TNFa, while the control group were not detected in supernatants of TNF. Show that the cationic liposomes prepared by directly transfected in vivo peritoneal cells and can express the gene product activity. 3 Discussion cationic liposomes as a new member of the family, is mediated in vivo transfer of genetic material an effective system. Cationic liposomes of neutral phospholipids and one or more of the positive ingredients,[link widoczny dla zalogowanych], and the interaction between the negatively charged DNA, thereby shrinking the DNA synthesis of compact structure,[link widoczny dla zalogowanych], the formation of a DNA liposome complex without requiring DNA sealed in resin vesicles within the plasmid, thus improving the protection of DNA, and to promote interaction with the cells and the expression plasmid condensation. Posed by the DOT-MA liposomes can spontaneously form complexes with DNA. FELGNERJ with the DOTMA and D () PE composed of liposome pSV2CAT to a variety of cells,[link widoczny dla zalogowanych], the results show that 101 * cationic liposome-mediated gene transfer efficiency of calcium phosphate precipitation is 5 to 10 times. DOPE is a medium-sized lipid molecules, called secondary fat, FAR-HOOD [] of the DOPE in cationic liposome DNA transfection role in the process, their DNA liposome complexes in the transfected cells at the same time, joined by. DOPE / DCchol blank consisting of cationic liposomes, the transfection efficiency was found to increase 8 times the maximum,[link widoczny dla zalogowanych], and this function and D () PE of the content. Yang and other cationic liposomes in vitro gene transfer into the ovarian cancer cells were obtained and stable expression of biological characteristics model, confirming the cationic liposome transfection works well J. In this study, cationic liposomes can be prepared in vitro to TNFa gene into NIH3T3 cells by G418 screening positive clones can be produced by expression of active product, the package TNFa gene after intraperitoneal injection of liposomes, cells in the peritoneal detected in culture supernatants in vitro TNFa, showed that intraperitoneal liposome-mediated pathway in vivo direct gene transfer. Liposomes as drug carrier selectivity, target specific, which can reduce the drug dose and reduce drug toxicity, in itself also has non-toxic, non-immunogenic characteristics, their toxicity in vitro transfected cells than calcium phosphate precipitation much smaller L6]. The results showed that the liposomes prepared in this experiment will be effective in vitro gene transfer into target cells, and directly through the abdominal cavity of cytokine gene transfer to peritoneal cells, the expression of active products. For further study of this research laid the foundation for gene therapy [
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